美国 Michigan State University 的 Robert Y. Ofoli教授来访

    应材化学院李伯耿院长的邀请，美国 Michigan State University 化工与材料科学系的 Robert Y. Ofoli 教授将于2007年10月22日访问本院，作学术报告，并介绍 Michigan State University 研究生的招生情况。

报告题目： Dynamics of a chromophore embedded in vesicles prepared by extrusion and by bath sonication

报告摘要： Bilayer lipid membranes are used extensively as a means of understanding biological systems at the molecular level. They are usually assembled by rupture of liposomes prepared from phospholipids, most commonly by extrusion or bath sonication. We used physical and optical characterization to compare liposomes formed by the two techniques, with the goal of determining whether the properties of the resulting liposomes (as well as bilayers reconstituted from them) differ at the molecular and mesoscopic levels. We used the phospholipid 1,2- dioleoyl- sn -glycero-3-phosphocholine (DOPC), with and without cholesterol, to form liposomes, incorporating 1- oleoyl- 2- [12- [(7- nitro- 2-1,3- benzoxadiazol- 4-yl)amino]dodecanoyl]- sn -glycero-3-phosphocholine (18:1-12:0 NBD-PC) as an optical probe of dynamics. We measured the physical morphology by transmission electron microscopy (TEM) and dynamic light scattering (DLS), and the rotational and translational diffusion of the probe by time correlated single photon counting (TCSPC) and fluorescence recovery after pattern photobleaching (FRAPP), respectively. Our results indicate that, in spite of differences in average size and size distribution, the two methods of preparation produced liposomes that exhibit the same molecular scale environment. The translational diffusion behavior of the tethered chromophore in planar bilayer lipid membranes reconstituted from the two types of liposomes also yielded similar results.

        报告时间： 2007年10月22日（星期一）上午10:00

       报告地点：玉泉校区 教十 2104

    研究生的招生情况介绍会时间：2007年10月22日（星期一）下午1:30

 

    

    化学工程国家重点实验室（浙江大学）
2007年10月17日